Molecular Formula | C15H24N2O |
Molar Mass | 248.36 |
Density | 1.03±0.1 g/cm3(Predicted) |
Boling Point | 352.6±37.0 °C(Predicted) |
Solubility | DMSO: ≥ 31 mg/mL |
pKa | 10.48±0.10(Predicted) |
Storage Condition | -20℃ |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 4.026 ml | 20.132 ml | 40.264 ml |
5 mM | 0.805 ml | 4.026 ml | 8.053 ml |
10 mM | 0.403 ml | 2.013 ml | 4.026 ml |
5 mM | 0.081 ml | 0.403 ml | 0.805 ml |
biological activity | MS049 is a potent, selective and cytoactive dual inhibitor of PRMT4 and prmt6, the IC50 was 34 nM and 43 nM, respectively. MS049 reduced Med12me2a and H3R2me2a levels in HEK293 cells. MS049 was not toxic to HEK293 cells and did not affect their growth. |
Target | IC50: 34 nM (PRMT4), 43 nM (PRMT6) |
Cell Line: | HEK293 cells HEK293 cells |
Concentration: | 0.1, 1, 10 μM 0.1, 1, 10, 100 μM |
Incubation Time: | 20 hours 72 hours |
Result: | Reduced the H3R2me2a mark in HEK293 cells in a concentration dependent manner (IC 50 =0.97±0.05 μM). Reduced levels of cellular asymmetric arginine dimethylation of Med12 (Med12-Rme2a, IC 50 =1.4±0.1 μM) in HEK293 cells. |